MIB-1 immunohistochemistry was performed using tissue microarray, and MIB-1 labeling index was recorded as the percentage of positive tumor cells.
To provide an indication of whether shRNA-induced VGLUT1 knockdown occurred in a restricted area close to the intrahippocampal injection site, immunohistochemistry was performed using tissue obtained after completion of the full behavioral test battery (27 30 days after lentiviral vector administration).
Analysis of tissue sections was performed using tissue fixed in 10% formalin for 24 h, dehydrated, and embedded in paraffin.
Triplicate measurements were carried out for each condition and each measurement was performed using tissue from a pool of three rosettes.
All analyses were performed using tissue from the distal colon since this is the site of tumor formation in both CAC and APC models.
These studies were performed using tissue samples from both the primary tumor and their related metastases (93 liver, 84%; 17 lung, 16%).
Immunohistochemical detection of Runx3 in human various cancer cases including 3 esophagus cancers, 3 gastric cancers, 3 colon cancers, 3 rectum cancers, 3 pancreas cancers, 3 liver cancers, 3 lung cancers, 3 kidney cancers, 3 bladder cancers, and 4 uterus cancers was performed using tissue microarray (MBL Co. Ltd., Nagoya, Japan).
Culture was performed using tissue culture inserts (0.2 μm).
To examine CS1 expression in OSCCs, immunohistochemical analyses were performed using tissue microarrays of OSCCs.
Cell-based quantitative analysis was performed using Tissue Studio (Definiens, Parsippany, NJ, USA).
