The signature of an action is the traffic which has been generated when it was performed previously possibly from another state of the application (For example, in Fig. 7 the signature of clicking on P2 is {Rb1,Rb2}).
Other diagnostic tests were performed when indicated, as previously described [ 28, 29].
Gene knockout was performed as previously described., When necessary, empty integration plasmids were transformed into appropriate yeast strains to ensure that strains being compared have the same auxotrophic genotype.
All stool samples were cultured for Entamoeba species in Robinson's medium (8 ) within 6 hours of collection, and hexokinase isoenzyme analysis was performed when possible as previously described (9 ).
Detailed observation is necessary, and repeat CE or DBE should be performed when bleeding persists despite a previously negative CE results in patients with OGIB.
Antigen retrieval, when appropriate, was performed as previously indicated (Muzio et al., 2005), while endogenous peroxidase blocking reaction was obtained by incubating sections 20 min in methanol/H2O2 3% solution.
Plays are performed, and, when they are performed, changed.
Except when stated otherwise, dissection and recordings were performed as previously described (Cangiano et al., 2007, 2012).
