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Comparative analyses of the transcriptomes using rigorous deep sequencing methods from fetal and adult brains have not been performed previously, particularly with a focus on a HERV gene.
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Although it was known that succinimidyl thioethers could undergo ring-opening hydrolysis reactions, such a thorough analysis on their stability had not previously been performed, particularly as it related to local protein environment and thiol exchange.
When phenotypical testing failed to identify bacterial isolates, a 16S rRNA gene sequencing was performed as previously described [ 22] because of its particularly good accuracy for species designation.
3WJdB transcription was performed as previously described29.
Microarray analysis was performed as previously described56.
Immunocytochemistry analyses were performed as previously described11.
ChIP was performed as previously described13.
Chromatin immunoprecipitation was performed as previously described28.
RNA-seq was performed as previously described27.
T7EI assays were performed as previously described31.
Surgical procedures were performed as previously described.
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