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For the most part, the analyses of this phenomenon have been performed in artificial systems and using animal models.
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To stimulate the chewing conditions, mechanical aging was performed in artificial saliva at 37 °C, and the load was applied using a three-point flexure fixture at a 2 Hz frequency.
The experiments were performed in artificial CSF (ACSF) containing 2 mM MgCl2 and 2 mM CaCl2 at room temperature.
Recordings were performed in artificial cerebrospinal fluid saturated with 95% O2 and 5% CO2 at 28°C to 32°C.
These were performed in artificial tear fluid (ATF) with various acidities to simulate the acidic microenvironment which may occur in a milieu of chronic ocular inflammation.
For slice experiments, calibrations and training set solutions were performed in artificial cerebral spinal fluid (aCSF): 126 mM NaCl, 2.5 mM KCl, 1.2 mM NaH2PO4, 2.4 mM CaCl2 dehydrate, 1.2 mM MgCl2 hexahydrate, 25 mM NaHCO3, 11 mM glucose, and 15 mM tris(hydroxymethyl) aminomethane, pH 7.4 (all Fisher, Suwanne GA).
In order to investigate NFAT transactivating activity directly, experiments have been performed in vivo with artificial promoters where a high number of NFAT binding sites were coupled to luciferase as a reporter.
A human health risk assessment has been performed in relation to recreational activities on two artificial freshwater lakes along the river Meuse in The Netherlands.
Electrochemical studies were performed in acidulated artificial saliva (AS) solution containing 0.2% and 2% of sodium fluoride (NaF).
The present study was performed in three artificial earthen ponds at the Fish Culture Station located in the Al-Qassim region of Saudi Arabia.
Measurements were performed in an artificial ground water (AGW) supplemented with urea (2 g.l− 1) and equilibrated at controlled temperatures with a gaseous phase before bacterial inoculation.
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