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For HPV 40 discrepancy, the capture probe design may not have been optimal to allow for hybridization with the HPV 40 target.
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All wells were screened microscopically for the presence of T cells on a daily basis and a culture period of 6-7 dappearedared to be optimal to allow maximum numbers of T cells to migrate from the skin fragments.
A minimum correlation of 0.70 is usually considered necessary, and alpha coefficient values above 0.90 are optimal to allow for individual comparisons [ 19, 20] The study was approved by Forskningsetikkommitté Syd (South ethical committee) Karolinska Institutet.
It is worth mentioning that both the research program and the research design of this study are optimal to allow knowledge translation (KT) and application throughout the entire research process.
For T. versicolor haustoria we determined that 25 μm cryosections were optimal to allow efficient tissue release from the adhesive coated Star Frost LPCM slides coupled with maximum tissue harvest volume.
The homozygous collection was grown for 5 generations (approximately 10 hrs) and the heterozygous collection for 20 generations (approximately 40 hrs), as these periods of time have been found to be optimal to allow each collection to resolve growth differences [ 43].
The GC column oven cycle was optimized to allow optimal peak separation for benchmark human EBC samples.
Within this evidence-based approach, clinical trials which incorporate random allocation of participants to treatment groups are considered optimal to allow evaluation of the comparative effectiveness of interventions.
The Simon 2-stage design was considered optimal to allow for early termination of the study after stage 1 in case of lack of efficacy.
The authors point out that the relative orientation of codon and anticodon in this early complex would not be optimal but might allow transient probing of the mRNA codon.
It could be argued that in the present study the vaccine matched the A/H3N2 virus perfectly, while under field conditions the match may not always be optimal allowing sub-clinical infections to occur, which may induce heterosubtypic immunity despite vaccination.
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