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Once written consent had been obtained, data from both cases and controls were collected by means of an ad hoc written questionnaire.
For those patients withdrawing from the trial after written consent has been obtained, data obtained up until the point of withdrawal will be included in the final analysis.
Intraindividual variability was observed between biopsies, which was not consistently linked to the localization of the sites where biopsies had been obtained (data not shown).
Under the ethical approval that has been obtained, data collected via the portal, the neurology clinics and routine administrative sources can be anonymously linked using the SAIL methodologies provided that agreement to the portal terms of service (via the portal) and written informed participant consent (at the clinics) have been obtained.
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In a control (without ultrasonicated lipase) a 34% conversion was obtained (data not shown).
Upon the consent was obtained, data collection was conducted by a trained research worker using a structured data form.
Same result was obtained (data not shown).
These experiments have all been reproduced with X4-HIV-1 and very similar data were obtained (data not shown).
In addition, when HEK293 cells were used instead of HeLa cells, similar results were obtained (data not shown).
When caveolae were enriched from detergent resistant plasma membrane of hepatocytes the same results were obtained (data not shown).
In a secondary analysis of all malaria episodes (with parasites at any density), similar results were obtained (data not shown).
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