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Despite these important challenges of intratumoral, intermetastatic, intrametastatic and inter-patient variability, some important findings have been obtained by sequencing methods, primarily that more than 138 driver mutations identified to date can be divided into pathways involved in cell survival, cell fate, and genome maintenance [30].
In these three cases, the reads had been obtained by sequencing genomic DNA from several individuals.
Haploid sequences have been obtained by sequencing DNA diluted to less than a haploid genome per aliquot [ 1– 3].
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Complete coding sequences from genomic DNA are obtainable for 154 of these proteins; two others, with "Ns" in the genomic data, were obtained by sequencing RACE products.
Genotypes were obtained by sequencing.
Phylogenetic position of strains used in this work was obtained by sequencing the housekeeping gene rpoD.
This cDNA clone was subcloned and the sequence of human DOR was obtained by sequencing both strands with a two-fold coverage minimum.
An EF-1α sequence of 624 bp was obtained by sequencing randomly picked clones.
For each gene and taxon, multiple sequence fragments were obtained by sequencing with different primers.
The complete sequence of Tn 916 from SC84 was obtained by sequencing the PCR-synthesized fragments.
Allele sequences were obtained by sequencing the bands excised from gels.
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