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Contrary to what had been observed using cell lines or for the case of aggregated α-Syn, we found strong differences in the immune response generated by wild-type α-Syn and the familial PD mutants (A30P, E46K and A53T).
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Cytochrome-c release was observed using Cell Fractionation Kit from BD Biosciences [30].
The specific cleavage of GRP94 by calpain could also be observed using cell lysates prepared from human gastric cancer cells.
Furthermore, the specific cleavage of GRP94 by calpain could be observed using cell lysates prepared from human gastric cancer cells (data not shown).
As a control, little co-immunoprecipitation was observed using cell extracts expressing either Flag-DVAP or Flag-DVAP-P58S but not Myc-Sac1 (Fig. 1B).
In all cases, no conversion of 5-haloindoles was observed using cells without the pSTB7 plasmid.
Again, no effect was observed using cells overexpressing EGFP only.
Similar results were observed using cells pretreated with 30 mg/L EEP or 5 mmol/L PBA compared with ox-LDL treatment alone (Fig. 3b).
Notably, no enhancement of metastatic dissemination to organs of the peritoneal cavity was observed using cells expressing NCAM-ΔFN2 (Fig 6A C).
Similar findings have been observed using arterial endothelial cells.
Similar increases in adherent cells were observed using either cell-free media from PMA or Astragalus treated cells (Figure 3B).
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