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Mechanical induction of HIF-1α has also been observed in endothelial cells exposed to low shear stress, and in the myocardium in response to mechanical stress.
As previously described for tumor cells, diverse activities have been observed in endothelial cells.
Changes in Bcl-2/Beclin1 complex have been observed in endothelial cell autophagy when treated with anti-angiogenic inhibitors.
No significant expression of inducible or neuronal NOS has been observed in endothelial cells under normal physiological conditions.
This is supported by the observation that pericytes can secrete tenascin-C in vitro (data not shown), and tenascin-W transcripts have been observed in endothelial cells (39).
A positive relationship between cell contact and proliferation has also been observed in endothelial and smooth muscle cells [ 26] and in kidney epithelial cells [ 27].
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A similar temporal relationship was observed in endothelial cells exposed to high glucose.
In contrast, high levels of KLF4 expression were observed in endothelial cells of cortical capillaries and in cortical neurons (Fig. 4).
Mild immunoreactivity of eNOS was observed in endothelial cells of vessels in brain, heart and lung tissues.
Expression of key cell cycle regulators was diminished in the double knockout cells, and decreased DNA synthesis could be observed in endothelial cells during embryogenesis.
To test whether the translocation of STK35 to actin stress fibers could be observed in endothelial cells, we transfected endothelial cells with EGFP-STK35 plasmid alone or together with mRFP-CLP36 plasmid.
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