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Also, much more genetic variability could have been observed by sequencing the gene encoding for the attachment glycoprotein (G gene) as suggested by RSV and limited HMPV sequences (14, 15 ).
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Surprisingly, the T-allele is also the ancestral one, as it has been observed by sequence comparison between 20 eutherian mammals, and, according to our results, this allele would have a protective effect against hypercholesterolemia.
More than 10 transformants were generated for the library, and no significant sequence bias was observed by sequence analysis of individual clones.
These small, error-prone, contigs have been observed by others using PacBio sequencing (Silke Alt, Natalia Miguel-Vior, Zhiwei Qin and Thomas Scott, personal communications).
Polyamides have been shown to bind to their target sites in chromosomal DNA and both gain- and loss-of-function have been observed by targeting repeated DNA sequences in developing Drosophila embryos.
So93, NZM 217/94, and our two strains share only 2 of 43 identical repeated sequences that have been observed by spoligotyping.
We observed that indels in coding sequences were enriched for lengths that are a multiple of three (3n) (Fig. 2) suggesting a purifying selection against frame-shift in coding sequences as has been observed by Daetwyler et al. [ 16].
No other known viruses (including BKPyV) were observed by deep sequencing.
The variation in expression levels of these 3,438 genes without an estimable ExpLevmRNA can only be observed by deeper sequencing.
DHPLC has been commonly used for mutation screening of various genes as it is rapid, cost-effective and highly sensitive detecting 93 to 100% of mutations that were observed by DNA sequencing [ 47].
It is also important to note that some key regulatory events may be occurring at the post-transcriptional and post-translational levels that will not be observed by transcriptome sequencing.
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