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The lack of activity of a truncated attC had also been observed before when associated with aadA10.
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Differential regulation of APL_0443 has also been observed before; APL_0443 was up-regulated when A. pleuropneumoniae was cultured in a growth medium favouring biofilm formation [ 6] and in the presence of porcine bronchoalveolar lavage fluid [ 20] and was down-regulated in A. pleuropneumoniae attached to SJPL cells [ 21].
Why have they never been observed before?
Effects of temperature on AP properties have been observed before (24), generally reporting AP lengthening when lowering the temperature from body to room temperature (36, 51).
This behaviour of v-SNAREs has been observed before [ 24] and could be enhanced when overexpressed.
This phenomenon has been observed before.
Comparing these results to actual seismic data reveals similar rate failures when precursors are observed before real earthquakes.
In the best case, similar D1.3 production rates were observed before and after in situ separation when IDA- or triazine-functionalized particle types were applied.
A moment of silence was observed before the game.
A moment of silence was observed before the race.
This became quite obvious during the H5N1 outbreak, but it was already observed before when avian influenza A viruses of subtype H7, including HPAIV, were found to be transmitted to man.
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