Sentence examples for been modified to encode from inspiring English sources

Full-length human SUV420H1_v1, SUV420H1_v2 and SUV420H2 cDNAs (Mammalian Gene Collection; purchased from Open Biosystems) were subcloned into pEGFP-C1 (BD BioScience), pEGFP-N1 (BD BioScience), and pCI (Promega) that had been modified to encode an amino-terminal hemagglutinin-A epitope tag.

To express Flag-A51R, and Flag-tagged forms of CPXV/ECTV/DPXV/YLDV/MYXV A51R proteins in vertebrate cells, each p166 vector was SacII/ PacI digested and inserts were cloned into pCDNA3 (Invitrogen) that had been modified to encode the same MCS as the p166 vector using linker ligation and primers described above.

Unlike other mammalian species, aged rodents have not been reported to develop Aβ deposits unless they express APP genes encoding human Aβ sequences [ 12, 38] or harbor modified APP genes in which the exon encoding the Aβ domain has been modified to encode human Aβ [ 10, 36].

First, a human anti-peptide library was constructed by diversifying a scaffold: the human variable heavy chain (VH) germ line gene 3-23, wasch was fused to a variant of the human variable light chain (VL) germ line gene A27, in which L1 was modified to encode the canonical structure found in anti-peptide antibodies.

For HA tagging, pKD13 was modified to encode in-frame double HA tag after the kan cassette removal.

Vectors were modified to encode KLF4, cMyc (Obtained from Open biosystems) or reprogramming factors were co-expressed using 2A elements as shown in Figure 1.

In order to reduce the flag number, a single flag could be modified to encode several blocks, increasing the compression rate of those k-sequence datasets with regular serial dynamics.

In addition, pBF27N was modified to encode EryF, the hydroxylase required for the production of EB from 6-dEB, thus generating pBF27N2.

To express Kv3.3-EGFP fusion proteins, the plasmid was modified to encode zebrafish Kv3.3a splice variant 1 (GenBank Accession #HQ118212.1) with EGFP fused in frame at the C-terminus via a four-residue linker comprising alanine-glycine-glycine-arginine before the initiation methionine in EGFP.

Human ZIC1-5 explasmidn plasmid vectors were constructed in pcDNA3.1 vector (Invitrogen) that had been modified to contain three tandem hemagglutinin (HA) epitope tag-encoding sequences.

Taking advantage of this high level of conservation, yeast strains have been genetically modified to encode specific transcriptional reporters responding to CTXs exposure.