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Full-length T-bet cDNA was inserted into the pMig retroviral vector employing XhoI and EcoRI restriction enzyme sites, which had been modified by inserting Thy1 instead of EGFP downstream of the internal ribosome entry site (IRES) sequence.
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The TPMA skeleton was modified by inserting differently m-functionalised phenyl substituents.
For convenient his-tag removal, the bacterial irisin expression construct34 was modified by inserting the 3C protease cleavage sequence (LEVLFQGP) in the Nde I site.
The device consists of a subphthalocyanine (SubPc)/fullerene (C60) heterojunction that is modified by inserting one or two additional donor layers between SubPc and the anode.
The 26650 cells are modified by inserting a temperature sensor into the cylindrical hole of the jelly roll and regarding their number of tabs at the positive connection.
The interfacial structures, playing a critical role on the transport properties and the perpendicular magnetic anisotropy in thin films and multilayers, can be modified by inserting an ultrathin functional layer at the various interfaces.
In a first experiment an endothelin-A receptor (ETAR) probe is modified by inserting a trivalent linker, attaching an albumin affinity tag and labeling the conjugate with the fluorescent dye Cy 5.5.
In the proposed method, the block of the DCT coefficients can be modified by inserting new nonzero coefficients 1 or -1, or removing coefficients 1 or -1.
Because the colour table is modified by inserting virtual colours, the reclustering is necessary to ensure that the same cluster configurations are used in the embedding and extraction phase.
In the present work, the intelligent gelatinase-stimuli NPs which are modified by inserting the optimal gelatinase-cleavable peptide (PVGLIG) between polyethylene glycol (mPEG) and polycaprolactone (PCL) segment (mPEG-Pep-PCL) [32] were set up to deliver Sal.
The 5' end of this PCR fragment (3 kb) was cloned blunted while the 3' end was modified by inserting into the primer both XhoI and BamHI restriction sites.
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