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Each sample used six to eight day-old yw females that had previously been maintained on a normal or fasting diet for 48 hours.
Assessment of isoflurane sensitivity was also performed in Dbh heterozygous (n = 13) and null siblings (n = 10) that have been maintained on a hybrid C57BL/6J×129/SvCPJ genetic background [49].
By contrast, wild-type mice that had been maintained on a high-fat diet for 20 weeks showed no reduction in food intake or body weight in response to the 450 ng/h leptin infusion (Figure 3).
Unlike wild-type animals on a high fat diet, ob-norm mice that had been maintained on a high-fat diet also showed a robust increase in STAT3 phosphorylation in response to leptin (2.7±0.8 cells for vehicle versus 108±21 cells for leptin, p<0.01).
ob-norm mice that had been maintained on a low-fat diet were sensitive to leptin, and showed a similar reduction of food intake as their wild-type counterparts, (15.2±1.4 kcal/d for vehicle versus 12.1±1.1 kcal/d for leptin, p = 0.08) and losing approximately 10 percent of their body mass over the course of the 12-day infusion (−2.8±1.1% for control versus −11.6±1.0% for leptin, p<0.05).
The populations used in our study have been maintained on a 14 day discrete generation cycle for several hundred generations.
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All mice were maintained on a C57BL/6 background.
A private army with associates around the world cannot be maintained on a shoestring budget.
He was maintained on a milrinone infusion while awaiting liver transplantation.
Rats were maintained on a 12-h reversed light dark cycle (lights off at 0900 h).
Responses received were maintained on a secure, password-protected server.
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