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I then asked among these gene duplications that had been maintained in zebrafish, how many of these zebrafish gene pair contained different numbers of total identified domains from their mouse homolog.
Further comparative genomics studies report that at least 20% of gene duplicates have been maintained in zebrafish, often by divergence of regulation between the duplicate loci that imposes an evolutionary constraint on both genes [ 22].
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This preservation in function is maintained in zebrafish despite only sharing 41% homology with mice and 46% with human OPTN (Bosenko et al., 2004).
Using a gain- and loss-of-function approach, we found that the TNFRSF1A and TNFRSF1B signaling pathways must be balanced for endothelial cell development to be maintained in zebrafish.
While the predominantly epithelial and epithelial-derived cellular expression of mammalian s100 genes is maintained in zebrafish, the actual regulatory mechanisms responsible for this similar expression may be different.
Zebrafish were maintained in the Zebrafish Facility at the University of Maine, Orono.
We found also that nkx6.1 is maintained in adult zebrafish in ducts and CAC/terminal end duct cells, which we show still have the potential to give rise to endocrine cells in normal (non-diabetic) animals.
To determine if circadian expression of retinal transcription factors is maintained in adult zebrafish, we performed a qPCR analysis on adult eyes collected over two days of an LD cycle and one subsequent day of DD.
Fish were maintained in the IMCB zebrafish facility according to standard procedures.
Interestingly, RT-PCR determined that hadp1 expression is maintained in the adult zebrafish heart, unlike in mammals (data not shown).
Zebrafish were maintained in recirculating tanks following instructions from ' The zebrafish book' (Westerfield, 2000).
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