CamKII and PKC activation by Wnt-5a is maintained in higher organisms and is essential for invasion of cancer cells (Weeraratna et al, 2002; Dissanayake et al, 2007).
All fibroblasts were maintained in high glucose DMEM (Invitrogen) containing 15% FBS (Hyclone), 1% Glutamax (Invitrogen), 1% non-essential amino acids (Invitrogen), 1% penicillin/streptomycin (Invitrogen).
All fibroblasts were maintained in high glucose DMEM (Invitrogen) supplemented with 15% fetal bovine serum (FBS, Hyclone), 1% glutamax (Invitrogen), 1% non-essential amino acids (Invitrogen), 1% penicillin/streptomycin (Invitrogen).
AMPKα1/α2 double knockout and WT mouse embryo fibroblasts (mefs) [60] were maintained in high glucose Dulbecco's Modified Eagle Medium (DMEM) with 10% fetal bovine serum.
Human embryonic kidney 293T cells [22] were maintained in high-glucose Dulbecco's modified medium supplemented with 10% fetal bovine serum (FBS).
Early-passage (≤20) HEK293 cells were maintained in high-glucose DMEM (Invitrogen, Carlsbad, CA) supplemented with sodium pyruvate (Invitrogen) and 10% Fetalplex (Gemini Bio-Products, West Sacramento, CA).
SK-OV-3 and NIH-3T3 cells were maintained in high-glucose DMEM (Gibco, Grand Island, NY) with 10% FBS, 100 IU/ml benzylpenicillin, and 100 mg/L streptomycin.
These cells were maintained in high glucose Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% (v/v) fetal bovine serum, 100 units/ml penicillin/streptomycin and 2 mM L-glutamine (Sigma-Aldrich).
67NR cells were maintained in high-glucose (4.5 mg/ml) Dulbecco's Modified Eagle's Medium (DMEM, Sigma, St . Louis MO) supplemented with 1% non-essential amino acids (Invitrogen, Carlsbad, CA) and 10% fetal bovine serum (FBS).
Cells were maintained in high glucose DMEM supplemented with 2 mM L glutamine, 10% fetal calf serum and antibiotics (100 units/ml penicillin, 100 µg/ml streptomycin and 1∶100 Biomyc3 anti-mycoplasma antibiotic solution, Biological Industries, Beit HaEmek, Israel).
