Sentence examples for been maintained in differentiation from inspiring English sources

The mRNAs were diluted with Opti-MEM I medium (GIBCO) before using Lipofectamine 2000 (Invitrogen) to transfect them into myotubes that had been maintained in differentiation medium for 4 d; 1 µg mRNA was used per 3.5 cm dish of cells [48].

The 3T3-L1 cells were maintained in differentiation media for 3 days.

Control cells were maintained in differentiation medium.

ATDC5 cells were maintained in differentiation medium as previously described and seeded at 150,000 cells/cm.

Cells were maintained in differentiation medium without RA from day 8 till day 20.

Cells were maintained in differentiation media (DMEM supplemented with 2.5% horse serum) for five days prior to harvesting.

ASC were maintained in differentiation media for 10 days and visualization of adipocyte differentiation was performed with Oil Red O (Sigma).

Medium was changed daily, and upon reaching confluence, cells were maintained in differentiation medium for 6 days (DMEM supplemented with 2 % horse serum and 1× penicillin/streptomycin).

For the induction into MSC-like cells, iPSCs were maintained in differentiation medium (DM), which is an α-minimum essential medium (Life Technologies) supplemented with 10% FBS and 5 × 10−5 mol/L 2ME.

This is the first time that the Clinical Practice of EM has explicitly broken down multitasking into interruptions and task switching and this differentiation has been maintained in the 2013 update.

The cells were maintained in the differentiation medium throughout the entire time period of differentiation.