Subsequently, the Brink's collection was maintained by self pollination at the Maize Genetics Cooperation - Stock Center University off Illinois, Urbana-Champaign).
TM-1 was selected as it is considered the G. hirsutum genetic standard and has been maintained by repeated self-pollination since its release in 1970 [ 62].
The A. majus line Sippe 50 [ 11] was obtained originally from the IPK Gatersleben and maintained by self-pollination while the second wild-type line 165E was produced by several generations of self-pollination from line JI.98 [ 16, 32].
Tomato is an autogamous crop and the sequenced accessions were maintained by controlled self pollination.
T1 seeds were set by self-pollination.
The NIL with IR64 background was developed by self-pollination of a plant selected from BC4F2 population.
For the induced plants, seeds were obtained by self-pollination of each treated plant and the chromosome counting was also conducted in root tips.
GsAG1- and GsAG2-expressing T2 transgenic plants (homozygous) were obtained by self-pollination.
This was followed by self-pollination in Shandong province in 2011.
