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Rosid I and rosid II comparisons have been made by sequence homology between soybean marker sequences and Arabidopsis sequences [ 17].
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†Diagnosis was made by sequencing and analysis of the viral protein 1 coding region after RT-PCR.
Diagnoses were made by sequencing products obtained by PCR with primers for the 17-kDa protein (4 ), citrate synthase (4 ), and PS 120 protein (5 ) genes.
While greater discovery of rare transcripts can be made by sequencing normalized mRNA pools, sequencing non-normalized samples enables one to obtain valuable information about changes in gene expression [ 13].
The uniqueness of those haplotypes relies on a very low number of mutations, clearly demonstrating the difference that can be made by sequencing the whole mitogenome compared to a subset of the mitogenome only.
Synthetic long reads are made by sequencing bar-coded ∼10 kb genomic fragments with standard Illumina short-reads, performing a local assembly of these 10-kb fragments into synthetic long reads, and then feeding them into a standard assembler (Voskoboynik et al. 2013; McCoy et al. 2014).
Indeed, many analyses of conserved coding or noncoding sequences have been made by classifying a sequence as coding (or non-coding) following a comparison with protein databases [ 1, 17, 18].
Conformationally constrained peptide libraries have been made by grafting randomized amino acid sequences onto a rigid scaffold derived from natural proteins.
At the species level, the correct species designation for all 55 clinical isolates was made by rpoB sequence similarity search (sequence similarity >94.0%).
For assessment, we remove close homologues to the sequence for which a prediction is to be made (by a sequence identity above a threshold).
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