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However, if phosphorylation at T426 is labile, the phosphate group might have been lost during preparation of the proteins for mass spectrometry.
For organizational reasons, he reduced the introduction because time had been lost during preparation.
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In response to the first point raised, regarding discarded insoluble material, we would like to emphasize that to ensure no protein was lost during preparation of the lysates, whole cell extracts were prepared without clearance of the insoluble protein pool in all of our experiments (except for the fractionation experiments in Figure 4).
After the FT-IR and Raman spectroscopy measurements, the sample was lost during preparation for ToF-SIMS analysis.
We would also encourage curators, and especially preparators, to take care with exceptionally preserved specimens as they may contain hidden information that could be lost during preparation work if UV light is not employed.
Some cases were inevaluable because tissue sample was lost during preparation, and other biopsies could not be used because insufficient tumor was detected in the sample.
In the STEM images containing a single FMO, there is an apparent place for a second FMO, suggesting that some FMO is lost during preparation or the possibility of two distinct populations of the FMO/RCC complex.
Seven sample pairs were excluded on clinicopathologic grounds: 4 had neo-adjuvant therapy, 1 had metastatic disease, and 2 had recurrent disease; 3 sample pairs were lost during preparation; and 1 pair was excluded when the mass spectra did not meet normalisation criteria.
Quantification by SEM is often limited [ 25] secondary to problems of bacterial enumeration arising from bacterial agglomerates and matrix-embedded bacteria [ 25, 55, 60] and from the possibility that some of the biofilm structure will be lost during preparation [ 23, 63].
Notably, the high molecular weight PAHs were lost during sample preparation, causing low recoveries.
It can be ruled out that spore biomass was lost during sample preparation as biomass was not filtered but centrifuged.
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