Sentence examples for been knocked down using from inspiring English sources

To test this idea, we examined EE localisation in embryos where nmy-2 had been knocked down using RNAi.

We showed previously that nucleolar dominance is disrupted in leaves of mature A. suecica plants in which the HDA6 or HDT1 genes have been knocked down using transgene-induced RNA interference (RNAi) [14], [16].

Additionally, one should test transfectants that express normal levels of the gene [ 19] or cells where expression of the gene has been knocked down using si or shRNAs [ 20].

Expression of both homologues of LGI1 in zebrafish, lgi1a and lgi1b, has been knocked down using antisense morpholinos, demonstrating that the two genes have acquired distinct but overlapping developmental functions (Teng et al., 2010, 2011).

The culture media for the stably-transfected prostatic epithelial cell line si- PRKC-ζ -a -PC-3M the6, in which the gene PRKC-ζ -a had been knocked down using si-RNA directed towards the unique 21 nt sequence – 5′-GTGAGAGACATGTGTCGTCTT-3′ – contained within PRKCZ exon 1 (Yao et al, 2010), were also supplemented with 500 ng  μl−1 Geneticin (Sigma, Gillingham, UK).

An earlier successful illustration of this principle was the rescue of developmental defects in zebrafish by co-injection of spliced RNA for each of two lncRNAs, cyrano and megamind, whose precursor RNAs had been knocked down using morpholino antisense oligos (Ulitsky et al., 2011).

The analysis was performed on BJ cells, following 24 hours treatment with aphidicolin, in which the Cernunnos/XLF protein was knocked down, using siRNA against Cernunnos/XLF (Fig 2C, 2D).

Twenty-five putatIL13-regulatingTFsg TFs were knocked down using short interfering RNA (siRNA), of which seven were found to affect IL13.

All experiments were performed at least 3 times with triplicate samples.> Genes of interest were knocked down using small interference RNA (siRNA) transfection.

Zebrafish spp1 was knocked down using morpholinos or the CRISPR/Cas9 system.

NF1 expression was knocked down using two independent lentiviral shRNAs in two NF1-expressing patient-derived glioma propagating cells (GPCs).