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EMVs have been isolated using differential centrifugation methods [ 12, 13] from cultured supernatants and body fluids including cerebrospinal fluid.
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Mitochondrial and cytosolic protein fractions were isolated using differential centrifugation.
The mitochondria from the lupin seeds were isolated using differential centrifugation and Percoll separation of crude organelles [ 34].
Proteins were isolated using Differential Detergent Fractionation (DDF) [ 27] for each of the tissues except muscle and brain.
injection of 90 mg/kg P5 or vehicle, blood plasma was harvested 1 h later and nanovesicles were isolated using differential centrifugation.
Mitochondria were isolated using differential centrifugation and immediately incubated with 0.1, 1, 10, 50 and 100 μg LPS per mg mitochondria protein on ice for 2 and 4 hours.
Cells induced for 36 h were disrupted after short cytochalasin treatment (38); mitochondria were isolated using differential centrifugation and sucrose gradient purification and then lyzed and sonicated in high salt buffer (50 m m KH2PO4, pH 7.4, 1 m NaCl, 0.05% Triton X-100, 10 m m Imidazol, 7 m m β-mercaptoethanol).
Of the 11 genes that were not tested using promoter fusions, one (AGL61) had been isolated using a similar differential screen and its promoter activity in the female gametophyte had been described previously [ 7, 9].
Mitochondria were isolated using conventional differential centrifugation [16].
Mitochondria were isolated using standard differential centrifugation techniques [32].
Mitochondria were isolated using standard differential centrifugation techniques (Trounce et al. 1996).
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