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Usutu virus (USUV) has been isolated in several African and European countries mainly from mosquitoes and birds.
Since then this organism has been isolated in several countries [9], and is considered one of the most important causes of ovine infertility, with a significant economic impact on the sheep industry [10].
WNV is transmitted mainly by mosquito vectors, although it has also been isolated in several tick species (3, 5, 13 ).
The Beijing strain has also been isolated in several African countries (21 – 26 ), and an association between this strain and the emergence of drug resistance has been reported in South Africa (27 ).
Prn-negative strains have been isolated in several countries that have high coverage for vaccination but have not been shown to constitute such a high proportion of circulating B. pertussis (12, 13, 20, 21 ).
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Until now over 50 different suppressor (Su var)) or enhancer (E var)) mutants were isolated in several laboratories, but only a few of them have been characterized in detail.
Knockout mutations were isolated in several genes in which such alleles were not previously available.
Other pathogens were isolated in several cases such that the extent of its pathogenicity remains obscure.
This study shows that SARS-CoV can be isolated in several cell lines commonly used for diagnostic or research purposes.
68,000 and 45,000 of 7BS and 7BL arms, respectively, corresponding to 50 ng of DNA, were isolated in several batches.
The P5CS gene was isolated in several species, such as rice (Yoshiba et al. 1995), Arabidopsis (Igarashi et al. 1997) and tomato (Fujita et al. 1998).
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