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Transforming genes coding for selectable functions, such as adenine phosphoribosyltransferase or thymidine kinase, have now been isolated by utilizing transformation in concert with molecular cloning.
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Six novel meroterpenoids cochlearoids F K (1 6) were isolated by utilizing phytochemical approaches.
These findings indicate that the fibroblast cultures derived from HGPS subjects contain naïve stem cell precursors that can be isolated by utilizing the SKP culture conditions described above.
PBMCs were isolated by use of Ficoll.
Transduced cells were isolated by FACS utilizing fluorescent protein reporter expression.
RNA utilized for these assays was isolated by means of a two-step extraction process.
Each monomer was isolated by column chromatography.
bYields were isolated by column chromatography.
C. gloeosporioides was isolated by sub-culturing.
Total RNA was isolated by TRIZOL Reagent (Invitrogen).
Proteins were isolated by the biotinylation assay.
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