Sentence examples for been isolated by limiting from inspiring English sources

To confirm our observation on a more stable population of transfected cells, 26 clones have been isolated by limiting dilution.

For each transfection, clones were isolated by limiting dilution cloning and screened by PCR for correct vector integration.

Parasites resistant to pyrimethamine were isolated by limiting dilution cloning and were subsequently screened by PCR for the loss of AMA2 gene using primers listed in the SI.

Single clones were isolated by limiting dilution and screened by immunoblotting.

I9-2a, b, d and e cells were isolated by limiting dilution experiments from I9-2 cells as previously described [13].

Clonal populations were isolated by limiting dilution into multiwell plates, followed by microscopic examination to identify wells receiving a single cell.

After transduction of the cells with the lentiviral reporter pRRL.SFFV.AniI-RS-GFP, clones were isolated by limiting dilution and fluorescence-activated cell sorting (FACS).

Selection began 48 h post transfection using 400 µg/ml G418 in DMEM, and subcolonies were isolated by limiting dilution and expanded into cell lines maintained in the selection medium.

Hybridomas were screened by enzyme-linked immunosorbent assay (ELISA), immunoblotting and immunofluorescence microscopy, and two positive clones (16G2 and 378F1, that gave identical reactivity by immunoblotting) were isolated by limiting dilution, and were used as undiluted culture supernatants for immunofluorescence, and diluted 1∶100 for immunoblotting.

Following selection, stable clones were isolated by limiting dilution.

Stable cells were selected with blasticidin and several clones were isolated by limiting dilution.