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Alternatively, the essential parasitism genes have not yet been isolated and functionally tested in ways that can truly reveal their function.
In previous studies, only 12 maize bZIP transcription factors have been isolated and functionally characterized.
In this study the phytoene synthase gene from C. zofingiensis has been isolated and functionally characterized.
So far, only a limited number of VvbZIPs have been isolated and functionally characterized [ 37].
Over recent decades, several enzymes in the process of strictosidine biosynthesis in C. acuminata have been isolated and functionally identified.
However, only few QTLs have been isolated and functionally analyzed, and few genes have been identified [ 5].
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Alytes obstetricans was recently investigated and novel peptides with antimicrobial activity were isolated and functionally characterised.
In this study, the rice UBC13 gene was isolated and functionally characterized.
Two PTHR homologues of the vertebrate PTH1R and PTH3R were isolated and functionally characterized in chicken.
These clones representing different gene clusters were isolated and functionally classified based on the MIPS standard [ 29].
Candidate gene alleles associated with positive or negative trait values can be isolated and functionally characterized in heterologous model systems such as yeast, Arabidopsis or tobacco [ 38, 57- 60].
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