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Similarly, MSC obtained from young individuals have been induced to undergo neuroectodermal differentiation in vitro, but this effect could not be reproduced in BMSC from elderly individuals [ 27].
The percentage of cells that have been induced to undergo apoptosis was determined by subtracting the percentage of apoptotic cells in the untreated population from the percentage of apoptotic cells in the treated population.
NGF treatment promoted a rapid reduction in the level of the p17 subunit of active caspase-3 in PC12 cells that had been induced to undergo apoptosis by various cytotoxins.
Notably, they were detected as cleavage products and/or were found in smaller amounts when the cells had been induced to undergo apoptosis than when apoptosis had been inhibited by z-VAD-FMK.
We investigated whether the newly identified PKs that were substrates of CASP3 were cleaved in HeLa cells that had been induced to undergo apoptosis by TNF α plus cycloheximide (TNF α) or anti-Fas antibody (anti-Fas).
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Almost 88% of the activated CD4+ T cells isolated from control MRL+/+ mice at the 4-week time period were induced to undergo activation-induced apoptosis in vitro.
Consistently, primary basal cells can spontaneously or be induced to undergo 'neural' development in vitro, generating NSC-like cells.
Finally, MSCs were induced to undergo osteoblastic differentiation on the three materials, and deposition of a mineralized matrix was quantified.
Somatic cells can be reprogrammed to form embryonic stem cell-like induced pluripotent stem (iPS) cells by forced expression of a set of transcription factors1, indicating that terminally differentiated cells can be induced to undergo cell fate change.
Human CD34+ HSPCs were induced to undergo erythroid differentiation after stimulation with pro-erythropoietic factors erythropoietin (EPO) and stem cell factor (SCF), this provides a valuable tool to study human erythropoiesis in vitro10,11.
NIH3T3 cells expressing GFP with a mitochondria-localization signal were induced to undergo mitophagy by expression of Park2 with or without treatment in the presence of 10 µM CCCP for 48 h.
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