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This indicated that proliferating precursor cells had been induced to differentiate into neurons.
All neurite outgrowth measurements were performed on cells that had been induced to differentiate for a total of 30 days.
However, the plasticity of MSC is not limited to mesenchymal derivatives, since MSC have been induced to differentiate into mesodermal, neuroectodermal and endodermal lineages [ 1].
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Confluent preadipocytes were induced to differentiate using a standard differentiation medium (DM2-L1, Zen-Bio Inc, Research Triangle Park, NC, USA).
Confluent preadipocytes were induced to differentiate with a standard differentiation medium (DM2-L1; Zen-Bio Inc., Research Triangle Park, NC, USA).
NSC cultures were induced to differentiate for 3 weeks in NSC differentiation media.
HUES-9 NSC cultures were induced to differentiate for 3 weeks in neuron differentiation medium.
HBM-MSCs were induced to differentiate into adipocytes, chondrocytes, and osteocytesusing a differentiation protocol as described previously [ 7].
The putative CSCs were induced to differentiate into different lineages using adipogenic, chondrogenic, and osteogenic differentiation media (PromoCell, Heidelberg Germany).
HBM-MSCs were induced to differentiate into adipocytes, chondrocytes, and osteocytes using a previously described differentiation protocol [ 20].
Upon characterization, the cells were induced to differentiate into smooth muscle (SM -like cellSM -likea variety of inducellsombinations.
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