Protein adsorption is a complex process, and the variety of involved physical mechanisms can hardly be included in binding isotherms that are used for practical applications.
In competition assays, a 50-fold excess of unlabelled NF- κB consensus or its mutant (Santa Cruz Biotechnology, CA, USA) was included in binding reactions.
This paper describes the Java MPI bindings that have been included in the Open MPI distribution.
Therefore a TIP-1 mutant protein (H90A) with abolished PDZ ligand binding capability [17] was included in the binding assay.
A stringent washing step (RIPA buffer) was included in our binding assay to reduce non-specific binding of actin to agarose beads (Fig. 1C).
To assay the binding ability of Fur in its apo form, EDTA chelator was included in the binding mixture at a concentration of 1 mM.
A great range of textures is included in this binding system, from terry pile toweling and corduroy to silk velvets and Oriental rugs.
To be effective, detailed climate change vulnerability and adaptation maps should be included in the binding landscape management plans.
For competition analyses, 50-fold excess of unlabeled competitor probe was included in the binding reaction.
This shift complex was specific for Hnf4α interaction as demonstrated with the formation of a supershift complex when Hnf4α antibodies were included in the binding reaction but not when substituted with control antibodies (Figure 6A).
In contrast, addition of SpxR60E negatively affected the DNA-αCTD-wild-type Spx complex (Figure 7A, lane 13), and completely abolished the DNA-αCTD binary complex (Figure 7A, lane 14) when it was included in the binding reaction with the G52R protein (compare with Figure 7A, lane 11, a reaction containing the G52R mutant protein alone with αCTD).
