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Finally, sceptrin's effect on cell motility could be mediated by its anti-muscarinic activity, as muscarine receptors have been implicated in migration in keratinocytes (28, 29).
Three of the new direct targets of miR-200c that we identify, MSN, FN1, and ARHGAP19, have been implicated in migration and invasion.
To determine the status of relevant GSK-3 targets, we conducted western blot analysis of GSK-3 substrates that have been implicated in migration regulation.
Fascin has been implicated in migration and invasion in vitro, so it was surprising that fascin loss had no effect on invasion in vivo.
Changes in the phosphorylation status of DCX and CRMP-2 have been implicated in migration control, raising the possibility that reduced functions of these proteins toward microtubules are responsible for the effects of Gsk3 deletion.
Furthermore, the co-regulation of ENO1 with T1α in the context of differentiation as well as induced injury is intriguing, because T1α is not only a marker for ATI cells in the adult lung but has also been implicated in migration and wound repair in the skin (Krishnan et al., 2013; Honma et al., 2012).
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An alternative explanation is that hepatoblasts in the liver-forming region migrate posteriorly upon Wnt8a overexpression since Wnt/β-catenin signaling is implicated in migration in other tissues (Yook et al., 2006; Aman and Piotrowski, 2008).
FLNa, the founding member of the family, was implicated in migration by cell biological analyses and the identification of FLNA mutations in the neuronal migration disorder periventricular heterotopia.
Tissue plasminogen activator, localized to the cell surface, is implicated in migration of tumour cells [ 21], whereas membrane type 1 MMP stimulates migration of MCF7 cells [ 22].
PLD2's lipase activity is implicated in migration and invasion of lymphoma cells via focal adhesion kinase (FAK) activation (Knoepp et al., 2008).
Finally, we show that SynIII phosphorylation at the CDK5 site is induced by activation of the semaphorin-3A (Sema3A) pathway, which is implicated in migration and orientation of cortical pyramidal neurons (PNs) and is known to activate CDK5.
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