The test organisms were grown in selected broth for 24 h.
The actin alleles (Table S4) were integrated into the disomic strain and cells were grown in medium selecting for the disome and plated on medium containing cycloheximide.
To isolate such a mutant, the TK-deficient cell line igm692-R1, in which the gpt gene had been inserted 3' of the endogenous μ gene by gene targeting, was grown in thioxanthine to select Gpt-deficient cells and thus enrich for μ-deficient cells [ 6, 13].
They were grown in synthetic selective media lacking uracil to select the URA3 gene on plasmid pUB23 (kindly provided by Dr. Alfred Goldberg).
Similarly, four to eleven lines from each of four combinations of homozygous FAD2-1A and FAD2-1B genes from population 4 were grown in Columbia MO and selected lines from population 4 were grown in Portageville, MO in 2009.
Cells were grown in media (SD His Leu) selecting for the cotransformed vectors containing RFA1 and RFA2 alleles.
Because aneuploidy increases genomic instability including higher rates of chromosome loss, disomic strains are grown in medium that selects for the presence of the duplicated chromosome ('Materials and methods').
T. thermophilus HB8 cultures were grown in the presence of the selected carbon source at 75°C.
E. coli was grown in LB and transformants were selected in LB plus 100 μg/ml carbenicillin.
Yeast strains selected were grown in clear bottom black 384-well plates (Costar) with SD medium for 4 6 h at 30 °C to reach early exponential growth (OD600 about 0.2 to 0.4).
