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Some food products have been grown in artificial environments to increase productivity.
Despite this change of practice and the acknowledged need for realistic field conditions to produce material for evaluation, numerous subsequent tests have been published where again biomass from glyphosate-tolerant cultivars is used for comparison despite having been grown in artificial conditions without application of complimentary herbicides.
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To check for the potential effect of nutrient depletion on the survival of polyps, via nutrient limitation of symbionts, replicates were grown in artificial seawater without soluble nitrogen and phosphorous (treatment of absence of nutrients).
Unicells were grown in artificial seawater until they reached the desired developmental age.
In summary, cell cultures of strain RCC745 were grown in artificial seawater and were naturally synchronized to a 12-h light/12-h dark cycle.
C. taxifolia was grown in artificial seawater at 25°C under fluorescent lights that provided PAR of 80 μmol of photos/m/s.
N. oculata was grown in artificial seawater supplemented with nitrogen and phosphorous under fluorescent lights that provided PAR of 40 μmol of photos/m/s to log phase.
Amaranthus hypochondriacus var RI03, Flaveria bidentis, Setaria viridis, and Arabidopsis plants were grown in artificial soil in a greenhouse, or in a growth chamber in soil or on media (see below) using growth conditions described previously [ 56, 67, 93].
Obligate intracellular bacteria cannot be grown in artificial media (agar plates/broths) in laboratories but require viable eukaryotic host cells (e.g., cell culture, embryonated eggs, and susceptible animals).
Maize (Zea mays) lines containing the rlsb-insertion (rlsb-1/ rlsb-2, rlsb-1/+ and rlsb-2/+), and sibling lines lacking the rlsb Mu insert (RLSB/RLSB), as well as wild type maize line B73 [ 32] were grown in artificial soil in a growth chamber (14 h/d illumination at 170 200 mmol photons m-2 s-1, with 22°C day 19°C night temps).
Seedlings were grown in an artificial climate chamber with 16 h light and 8 h darkness (22 °C) with 70% relative humidity.
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