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Of the 44 species they identified as plants they wanted to cultivate a few short months ago, 15 are now thriving in the garden; the seeds have been germinated using data collected in trials at Kew, and because they are native to this place they need little or no irrigation.
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Seeds were germinated and DNA was extracted using a standard CTAB protocol [ 27] with modifications, as described in [ 28].
Imported GMOagricultural products for consumption (e.g. corn and soybean) are allowed unless the products are germinated and unless they can be used as seeds.
In Uganda, a thin porridge called obushera is popular; it is prepared by using germinated fermented grain flour.
After 7 days, the germinated and non-germinated seeds were counted using a binocular microscope.
Number of germinated seeds were analysed using the spreadsheet of Apache OpenOffice.
The germination index was calculated using the following formula: Seed Germination Index = Number of germinated seeds Total number of seeds × 100.
The genomic DNA from all eleven germinated T1 plants was evaluated using PCR.
dRate of germination (RG) was calculated using the following formula, RG = ∑ Ni/Di where Ni is the number of germinated seeds in a given time, and Di is the time unit (day).
Seeds of both JG62 and WR315 were sterilized using 0.1% HgCl2 and germinated in autoclaved sand and synthetic soil mixture (1∶1).
The proportion of germinated seeds, normal seedlings, the time to reach 50%% of the maximum germination (T50) and the statistical significance were calculated using the software program Germinator (Joosen et al., 2010).
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