The Colorectal DSA was developed based on the colorectal transcriptome, which was generated from large-scale in-house sequencing, public data mining and experimental investigation [ 32].
dbEST contains more than 100,000 A. gambiae ESTs, the majority of which were generated from large-scale sequencing of two non-normalized cDNA libraries constructed from non blood-fed and blood-fed whole adult females [ 1]. 601, or 66.9% of the 898 unique gene products sharing identity with the genome but not identified by BLASTX search of GenBank Nr were identified by this BLASTN search of dbEST.
The vast amount of oncogenomics data are generated from large scale collaborative cancer projects (Table 5).
Primary data from the current study (RNA-seq and LC-MS/MS) were compared and integrated with data generated from large-scale proteomics projects [ 16, 18, 45, 46], as well as with gene expression datasets deposited within the GEO database [ 47].
However, the conventional simulation and analysis methods are becoming inadequate when dealing with such 'big data' generated from large-scale DNS.
In this study, the RNA-seq method was adapted for small-scale samples to yield RNA-seq data at a level comparable to that generated from large-scale samples (supplementary material Fig. S2).
After applying this method to cancer gene mutation data generated from large-scale and whole genome sequencing of cancer samples, a network of cancer genes with co-occurring and anti-co-occurring mutations was constructed.
The gimli mutation was generated from a large scale EMS-mutagenesis screen for mutations affecting salivary gland and tracheal development (Myat et al., 2005).
Such models can be generated from any type of large-scale datasets, and are usually completed with building information that can be derived from several interaction databases.
Further, transgenic goats were generated for large-scale production of the fusion protein.
