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To exclude mono-tags that may have been generated by sequencing errors, only those represented in at least three different libraries and observed 22 times or more across all libraries were analyzed further.
Recently, an ordered draft sequence of the 17Gb hexaploid bread wheat genome has been generated by sequencing isolated chromosome arms and major efforts have been undertaken worldwide to sequence and annotate the wheat genome [ 21– 28].
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Sequencing of the round 3 sub-library generated a greater percentage of location-collapsed HBV reads than were generated by sequencing the corresponding initial library (Table 1).
Over 4.8 billion reads were generated by sequencing the samples.
In total, about 20,000 high-quality BAC end sequences (BESs) were generated by sequencing 15,000 clones.
Most of the data in the present study were generated by sequencing only one sample.
Paired-end 76 bp reads were generated by sequencing using the Illumina HiSeq instrument.
The porcine ITGAV coding sequence was generated by sequencing ITGAV specific PCR products amplified in lung cDNA.
The genotypes for the missing data were generated by sequencing the DNA segment containing the polymorphism locus.
Some of these sequences may be isomirs, as some nt variations occur at the 5′ ends or internal sequences, which are unlikely to be generated by sequencing errors.
A total of 1 004 081 raw sequencing reads with an average length of 516 bp were generated by sequencing 6 cDNA libraries.
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