Sentence examples for been generated by inserting from inspiring English sources

Since pMT-LacZ has no NheI site, this site had been generated by inserting a double stranded oligonucleotide containing an NheI site into the KpnI/SpeI sites of the vector.

The mammalian CSB expression plasmid used in the TAP technique (pZome-1-N-TAP-CSB) has been generated by inserting the full-length coding region of human CSB cDNA into the BamHI site of pZome-1-N (Euroscarf, Heidelberg, Germany).

In this study, wall roughness is generated by inserting a Shchelkin spiral with different wire diameter and pitch (Ls).

For the rule given in Figure 4, catechol was provided as an initial seed pattern, and various other structures were generated by inserting every type of bond atom pair into every position in the seed.

The donor plasmids, such as pFa-Cap, pFa-BS-Cap, pFa-Cap-HS4, and pFa-Cap-HS4−, were generated by inserting the amplified PCV2 ORF2 gene into pFastbachtb, pFa-BS, and pFa-HS4.

The plasmids of pGEX-6P-1-GST-FXYD1 to pGEX-6P-1-GST-FXYD5, pET28a-His-FXYD6 and pcDNA3.1-myc-FXYD6 were generated by inserting the corresponding full length cDNAs into the empty vectors.

pFlag-A22-2UIM-M1G was generated by inserting the 2UIM-encoding 3' region, as described above.

The GNL1-GFP construct was generated by inserting the GNL1 coding region into p068.

Bra>Eph4ΔN, Bra>Eph4TM and Bra>Eph3ΔN were generated by inserting PCR-amplified cDNAs using SalI and BamHI.

Ndk-DsRed plasmid was generated by inserting PCR-amplified Ndk between the XhoI and HindIII sites of pDsRed2-N1 (Clontech).

The HA-Smad4 plasmid was generated by inserting the PCR product of Smad4 into a pcDNA3 vector [20].