Sentence examples for been generated by directed from inspiring English sources

Affibodies are a group of affinity proteins that are based on a 58-amino-acid residue protein domain derived from one of the IgG-binding domains of staphylococcal protein A. A single human IgA affibody with high IgA affinity has been generated by directed evolution.

Cardiomyocytes have also been generated by directed differentiation with Activin A and BMP4 on a dense monolayer of hESCs; these cells successfully form functional cardiomyocytes when transplanted in vivo [ 51].

Moreover, highly performant red fluorescent proteins were generated by directed evolution of mRFP, which is itself a monomeric derivative of the DsRed protein [ 13, 14].

A mutant unable to hydrolyse ATP (K304A in the Walker A box) and a mutant designed to abrogate the nuclease activity (D10A/E12A) were generated by directed mutagenesis.

The all-Ala-CL Pgp only conferred partial resistance against valinomycin and none against FK506 and doxorubicin, demonstrating the value of the new CL-Pgp that was generated by directed evolution.

Plasmid pTTR210K encoding the mutant subunit K401-BIO-H6 K401-BIO-H6 K401-BIO-H62 by diR210Kd mutagenesis (QuikChange, Stratagene) of the R210 CGA codon to AAA. pTTSTOP, wasch encodes untagged wild-type K401, was generated by directed madegenesis ofromdon 402 (CGC) of pWC2 to the stop codon TGA.

Microstructures of poly N-methylpyrrole) have been generated by direct electrochemical oxidation of N-methylpoly N-methylpyrroleyrenesulfonic acid) in aqueous solution, using a micelle formation mechavesm with gas beenle templates.

Recently, iPSCs have been generated by direct delivery of recombinant proteins, RNAs, or microRNAs[57 59].

Although two-component biomolecule patterns have been generated by direct-write DPN [52, 84], further equipment modification and operation will be required for fabricating extensive multicomponent arrays.

More recently, induced pluripotent stem cells (iPSCs) have been generated by direct reprograming of differentiated cells (Takahashi & Yamanaka, 2006; Buganim et al, 2013).

To reconstruct the sequences that would have been generated by direct sequencing of diploid PCR products from these five loci (i.e., with ambiguity codes), the two alleles from an individual genotype were collapsed into a consensus sequence using MESQUITE v2.5 [ 42].