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Microstructures of poly N-methylpyrrole) have been generated by direct electrochemical oxidation of N-methylpoly N-methylpyrroleyrenesulfonic acid) in aqueous solution, using a micelle formation mechavesm with gas beenle templates.
Recently, iPSCs have been generated by direct delivery of recombinant proteins, RNAs, or microRNAs[57 59].
More recently, induced pluripotent stem cells (iPSCs) have been generated by direct reprograming of differentiated cells (Takahashi & Yamanaka, 2006; Buganim et al, 2013).
To reconstruct the sequences that would have been generated by direct sequencing of diploid PCR products from these five loci (i.e., with ambiguity codes), the two alleles from an individual genotype were collapsed into a consensus sequence using MESQUITE v2.5 [ 42].
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Affibodies are a group of affinity proteins that are based on a 58-amino-acid residue protein domain derived from one of the IgG-binding domains of staphylococcal protein A. A single human IgA affibody with high IgA affinity has been generated by directed evolution.
Although two-component biomolecule patterns have been generated by direct-write DPN [52, 84], further equipment modification and operation will be required for fabricating extensive multicomponent arrays.
Cardiomyocytes have also been generated by directed differentiation with Activin A and BMP4 on a dense monolayer of hESCs; these cells successfully form functional cardiomyocytes when transplanted in vivo [ 51].
First generation MHC microarrays were generated by direct spotting of the pMHC of interest onto the microarray slide.
Induced pluripotent stem cells (iPSCs) are generated by direct reprogramming of somatic cells and hold great promise for novel therapies.
To do this, particle trajectories were generated by direct numerical simulation of the Navier-Stokes equations for the case of a turbulent flow.
Here we describe an alternative strategy for Parkinson's disease in which dopamine neurons are generated by direct conversion of astrocytes.
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