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Though the transcriptome sequencing for non-model organisms using NGS was almost confined to 454 pyrosequencing due to its longer read length compared with the other two platforms [ 10, 20, 25], it is noteworthy that a draft genome sequence for the giant panda has been generated and assembled successfully using only Illumina Genome Analyser sequencing technology [ 26].
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Genomic contigs were generated and assembled by using the Newbler assembler software (Roche Diagnostics, Basel, Switzerland).
Genomic contigs were generated and assembled by using the Newbler assembler software (Additional file 2: Table S3).
Briefly, from the previous study, 438,321 ESTs were generated and assembled into contigs and singletons [11].
Six Gbp of data were generated and assembled into unigenes.
The 454 reads were generated and assembled by Roche.
Biotinylated DR1-A12 molecules were generated and assembled into tetramers by using phycoerythrin-streptavidin.
A total of 374.6 Mb of data were generated and assembled into 83,898 unigenes.
Approximately 10.7 GB of data was generated and assembled into 37,843 unigenes.
More than 10 reads were generated and assembled into a 19.5-Mb genome.
74 million sequences were generated and assembled into 96,492 contigs using the Trinity software suite.
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