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The biological significance of the in vitro data has been further validated in vivo.
These genes have been further validated in the functional context of networks and exhibit high potential for understanding pathways and pathway crosstalk associated with T2DM.
Using an unsupervised approach, we have identified five clinically relevant subtypes of breast tumors [ 4, 8], which have been further validated in independent data sets [ 3, 9- 14].
Both the Amsterdam and Rotterdam signatures have now been further validated in large multicenter investigations that confirm the prognostic advantages of the expression signatures over conventional guidelines for selecting patients for adjuvant systemic therapy [ 8, 9].
These data have been further validated in GMG-ITC treated SOD1tg rat by a high and significative FoxP3 detection, as an indirect marker of Tregulatory (Treg, also known as CD4+/CD25high/FoxP3+) cell presence).
The findings did, however, spark an interest in the potential use of Gln as a GLP-1 secretagogue in human subjects, and it is notable that an effect originally identified in a cell line was subsequently reproduced in type 2 diabetic and obese subjects (10), and has now been further validated in studies on primary L cells.
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In an independent validation set, DKK3 and ITIH5 were further validated in combination with the known biomarker RASSF1A to increase sensitivity of the panel.
The psychosocial functioning scale utilised in the current study needs to be further validated in future research, as only internal reliability and internal validity was assessed.
This enrichment was further validated in the independent microarray datasets (Supplementary Fig. 25).
These inhibitors were further validated in a panel of six osteosarcoma cell lines.
These results were further validated in conventional cell culture platforms and within three-dimensional scaffolds.
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CEO of Professional Science Editing for Scientists @ prosciediting.com