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They have been identified as LigF-LigE-LigP and LigG, respectively, in SYK-6, and homologs have been functionally verified in closely related sphingomonads (34 – 37), including N. aromaticivorans DSM12444, which also contains genes present in the GGE dehydrogenase clade.
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Before Cre-mediated cassette exchange in a non-drosophilid insect was tested, all components were functionally verified in D. melanogaster, for which lox-site RMCE was previously demonstrated, but with the use of a P vector and markers specific to that species (Oberstein et al. 2005).
To date, two fungal LXRs have been functionally verified to be involved in l-arabinose catabolism, including the NADH-dependent ALX1 of the yeast Am. monospora(19) and the recently identified NADPH-dependent LxrA of A. niger.
In rice, only two PTR genes have been functionally verified.
However, the putative NHERF1 tumour suppressor activity has not been functionally verified.
ZmC1, the maize anthocyanin synthesis regulator that was shown to activate the Arabidopsis ANR promoter [ 11], clustered together in the same subgroup with AtTT2 and VvMYPPA2, which are functionally verified PA regulators.
The BbNN is designed as an system-on-chip (SoC), and is functionally verified and tested on several case studies.
All designs are carefully clocked and were functionally verified using QCA designer.
Their orthologues have also been annotated and/or functionally verified in a number of important crop species.
Then, it has been synthesised, functionally verified and implemented using Xilinx Integrated Software Environment (ISE) and Spartan-3E FPGA.
Also in the alignment were several functionally verified dehydrogenases from various organisms that were included on the basis of the same criteria for percent identity as described above (see Table S2 in the supplemental material).
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