However, only a handful of mutations in IRF6 have been functionally tested, therefore it is impossible to know the true sensitivity and specificity of these in silico prediction programs in this case.
Hence, although a predisposing role cannot be excluded until all associated SNPs have been functionally tested, true risk variants may need to be sought through an extended and comprehensive analysis of the NPSR1 locus, both at the genetic and functional level.
To date, all PREs that have been functionally tested bind Pho (Oktaba et al. 2008; Kassis and Brown 2013).
The role of many of these DAF-16 targets for longevity has been functionally tested; RNAi of many genes up-regulated by daf- 2 leads to decreased daf- 2 lifespan and RNAi of many genes down-regulated by daf- 2 leads to increased wild type lifespan (Murphy 2006).
Many of the members of this clade have been functionally tested (Additional file 1: Figure S1 and Table S1) and show conserved function across all species as H+- myo-inositol symporters [ 16, 21].
The developed algorithm has been functionally tested and validated with different structural settings of workflows with a number of critical cases.
Stbm contains potential interaction motifs for the endocytic adaptor AP2 [34], but their role has not been functionally tested.
Although these cells have not been functionally tested, the fact that CD39 is a marker of Tregs [ 78, 79] suggests that, like CCR6, adenosine could promote a shift from Th-17 cells into Tregs.
Many of the prohormones investigated here have not been functionally tested in the songbird; thus, neuroanatomical localization of prohormone expression is a useful guide for further examination of the function of neuropeptides in songbird biology.
Although not all of these binding sites in the Ndg enhancer have been functionally tested, mutating either the Ets site or the Tin site eliminates enhancer activity in the heart (Busser et al., 2012a) (supplementary material Fig. S12).
