To validate the microarray data by means of qPCR we selected the top 3 genes from Table 2 (highest average expression across WAT, LIV, and SM: Ddit4, Cdkn1a, and Cebpd), none of which have previously been functionally described in the context of fasting in mice, as well as Per1 and Fasn, known responders to food deprivation and thereby positive controls.
The archetypal Kunitz fold is highly conserved, resembling the first Kunitz-domain protein, the bovine pancreatic trypsin inhibitor (BPTI) that was functionally described in 1936 by Moses Kunitz [ 24].
The combined activities of these enzymes have been previously described in functionally-based cellulase models [ 2- 5], and the various approaches taken have been recently reviewed [ 6].
We were interested in pairs of amino acid sites that are either functionally important themselves or that are alternatively surrounding important functional domains (less than 8 Å distant from functionally important sites that have been described in other works, see Material and Methods for details).
Functionally, no motion abnormalities have been described in athletes despite having a lower deformation in the basal segment of the RV; abnormal motion of the RV is essential data to fulfil the ARVD diagnostic criteria.
In 30 years since editing was discovered in mitochondria of trypanosomes, several functionally and evolutionarily unrelated mechanisms have been described in eukaryotes, archaea, and viruses.
Neurons functionally specialized for nociception have been described in invertebrates such as the medical leech and the marine snail Aplysia californica (Walters 1996).
At least two phenotypically and functionally distinct monocyte subsets have been described in humans, rats, pigs, and mice (Geissmann et al., 2003; Yona and Jung, 2010; Ziegler-Heitbrock, 2014), indicating evolutionary conservation.
Functionally important small RNAs were first described in nematodes in 1993 [ 1, 2].
However, the absence of these key residues in functionally active ShKT proteins was already described in snakes [ 75].
The p.Ile35del variant for instance, was not predicted as functionally damaging; however, it is described in Fatima et al. and Saeed et al. to provoke obesity in homozygous conditions [13, 16].
