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Since most marine teleosts spawn pelagic eggs [85], and only three Vtg forms from marine pelagophils have been fully sequenced to date, we suggest that the present tree does not reflect the bias toward VtgAa type genes.
In Streptomyces, two species have been fully sequenced to date, S. coelicolor and S. avermitilis, and their genomic sequences were used for the prediction.
The human papillomavirus (HPV) family is a heterogeneous group of epitheliotropic viruses of which more than 100 genotypes have been fully sequenced to date (de Villiers et al, 2004).
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The resulting construct was fully sequenced to ensure the fidelity of the PCR.
Each of the constructed transgenes was fully sequenced to ensure no PCR error before being transformed into Agrobacterial cells.
Recombinant plasmid DNA was isolated and the SMP30 cDNA insert was fully sequenced to confirm its authenticity.
Coding regions in all constructed plasmids were fully sequenced to verify that no undesired mutations had been introduced by PCR.
The pfmdr1, pfcrt, pfmrp1 and pfmrp2 ORF were fully sequenced to determine the genetic variability between the clones.
The amplicons were cloned into pENTR/D-TOPO (Invitrogen) to generate gateway entry constructs, which were fully sequenced to ensure sequence integrity.
Both constructs were fully sequenced to confirm plasmid fidelity.
All cDNAs were fully sequenced to confirm their identities, and primer sequences are available on request.
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