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Centromeres are located near or within repetitive DNA sequences, but sequence specificity has only been found in budding yeast.
Several potential Cdk1 phosphorylation sites have been found in budding yeast Ku70/Ku80; however, their mutation did not affect NHEJ activity (Zhang et al., 2009).
Indeed, all the components of the RNAi machinery are present in fission yeast, whereas none of them have been found in budding yeast, although both genomes have been fully sequenced [ 43].
Mutations in other genes resulting in EST phenotypes, have so far only been found in budding yeast (EST1, EST3, CDC13) [118,119], worms (MRT-2) [120] and Arabidopsis (POT1A) [41].
Moreover, most plant miRNAs target the CDS but not the 3′ UTR of transcripts and no miRNAs have been found in budding yeast, suggesting that uncapped 5′-ends specifically accumulated 2 3 nt upstream of the PUF binding site are unlikely to be the products of miRNA-guided cleavage.
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Similar electron-dense bridges were found in budding and fission yeast cells, but they have not yet been attributed to a specific protein [9], [48], [49], [50].
A protein called Sup35, which is found in budding yeast, can exist as two different prion forms.
Temporary downregulation of E-cadherin was found in budding lobules invading the stroma of breast tissue [ 11].
In a more recent study, a severe nuclear chromosome instability (CIN) phenotype was found in budding yeast cells completely devoid of mitochondrial DNA (Veatch et al. 2009).
Of the four potential dihydroflavonol reductase (DFR) NRs, the most highly represented was found in bud and young fruit.
13 Although GLP-1 has been found in taste buds and brain tissues, the majority of the GLP-1 measured in peripheral blood is synthesized in L cells.
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