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Comparative analysis of related genomes confirmed that all of the IILs have been fixed during evolution.
Therefore, all of the intron losses that we observed in S. lycopersicum have been fixed during evolution.
Taking advantage of the availability of genome sequences corresponding to multiple A. thaliana lines, we also tested whether the intron variations that were observed in A. thaliana have been fixed during evolution.
Similarly, by surveying 104 PILs in 180 lines of A. thaliana, we found that 101 cases of PIL have been fixed during evolution and only three PILs remained polymorphic, in genes AT1G48420, AT3G23080, and AT4G00350.
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It would be optimal to compare gene expression between the two populations also at other ages, as this could help separate genes whose expression is affected factors such as load and relative differences in cell type abundance from those whose differential expression is caused by certain alleles having been fixed during domestication or evolution.
In other words, these two insertions were fixed during the evolution and divergence of the AA genome species.
In the former, apparently only a few mutations were fixed during the evolution of the modern species; accordingly, the Hamming distances between the clones are comparably small, as are the resultant PBC distances between the individuals.
The rotor was fixed during the measurement.
Packing density is fixed during the design of the field.
432 of these violations were fixed during these inspections.
Hearts were fixed during diastole using 100 mmol/L CdCl2.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com