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Recently, two new droplet-based RNA-seq technologies, named as Drop-seq and inDrop (indexing droplets), has been exploited to sequence in parallel thousands of single cells from a tissue [62, 63].
This has largely been exploited to sequence the coding, or exome, portions of the genome.
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In brief, the release of hydrogen ions on incorporation of a nucleotide into a DNA template has been exploited to determine the sequence of a piece of DNA (Rothberg et al, 2011).
In the present study the genomic resources of the zebrafish have been exploited to investigate the molecular sequences of events invoked under zinc depleted conditions.
Between-genome compositional variation has been exploited to assign environmental shotgun sequences to their most likely originating genomes, whereas within-genome variation has been used to identify recently acquired genetic material such as pathogenicity islands.
Moreover, the large body size of the silkworm has been exploited to establish multiple tissue-specific expressed sequence tag (EST) libraries [ 21, 22].
Since its first application few years ago [4 6], whole exome sequencing has been exploited to identify the causative gene of many monogenic disorders, including skeletal diseases.
To identify genes encoding PCWDEs, HMMER3 package [ 26] was exploited to build sequence profiles and predict putative genes.
However, we point out that the protein topology has a wealth of information, which can be exploited to design sequences for a chosen structure.
The same strategy could also be exploited to introduce sequences into an endogenous gene, creating a knock-in allele.
These properties can then be exploited to obtain a sequence of faded symbols where the primary purpose of the filter is to suppress multiuser interference and track the ratio between successive fading coefficients; thus, not burdening it with estimation of the fading coefficients themselves.
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