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Maraviroc demonstrates potent antiviral activity against all CCR5-tropic HIV-1 viruses tested (geometric mean 90% inhibitory concentration of 2 nM); its mechanism of action has been established using cell-based assays, where it blocks binding of gp120 to CCR5 to prevent the membrane fusion events necessary for viral entry.

Transduction profiles were established using cell type specific markers (for horizontal cells we used Prox1, and for ganglion and amacrine cells calretinin) comparing transduced retinas with and without collagenase treatment (Fig. 5A and B).

Nucleic acid sequence-based amplification assays were established using cell line RNA at the laboratories of Organon Teknika (Boxtel, The Netherlands).

Cultures were established using cells taken from single colonies grown on agar plates and inoculated into 2 mL of YPD in 5 mL snap-cap culture tubes.

Presence of TnTs in these cancer cells was established using cells derived from both human patient specimens as well as from established cancer cell lines.

With regard to the other aspects of the cellular and molecular effects of ET, most of the current knowledge has been established using renal cells and comparison of the data established using neurons (this study) and renal cells reveals several commonalities.

Once the optimum cell culture and flow conditions had been established using QV500 chambers, the cells seeded in the coated coverslips, further experiments were performed exploring the effect of Aβ25-35 Aβ25-35 cell type tongeach proof of celleptype toe multicellular co-culture system.

Its cellular source in activated microglia has been established using high-resolution, single-cell autoradiography with the R-enantiomer [3H](R -PK11195.

To test function in vivo, a human breast cancer xenograft model has been established using MDA MB 435 tumour cells engineered to coexpress MUC1 and firefly luciferase.

The antigen specificity of the T cell clone was established using these cells as effectors cells in a CTL assay.

The in vitro cell growth inhibition in differentially treated drug resistant human ovarian cancer cell lines was established using a cell-viability assay.

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