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Limited data are available for only a subset of taxa for which axenic cultures or pot cultures have been established from single spores (so-called "isolates") (Tisserant et al. 2012, 2013).
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Many plantations were established from single plants, shipped out to various colonies - a single plant was taken from Amsterdam's botanical gardens to Surinam in 1718, another was sent to Martinique in 1720, and so on.
Liquid cultures were established from single colonies.
Populations of targeting strains were established from single G1 positive adults by backcrossing to wild-type.
Transgenic strains were established from single G1 positive adults by backcrossing to wild-type (KIL strain).
These epithelial-like (Figure 1A) and fibroblast-like cells (Figure 1B) were expanded via limited dilution and individual cell clones were established from single cells.
Clonal cultures were established from single asexual females.
MA lines of PFM5 were established from single colonies isolated on Miller Luria-Bertani (Miller 1992) agar plates.
Stocks were established from single males bearing reconstituted P{CaryP}attP40 and P{CaryP}attP2.
At time T0 twenty lines were established from single cells all originated from the same founding clonal culture.
In vitro cultures of each isolate were established from single spores with Ri T-DNA-transformed carrot root on standard M growth medium [ 55].
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