Once randomisation is completed, the intervention team will enumerate all households in the intervention tabancas that had not previously been enumerated, and then invite the residents to take part in the intervention services.
Mice were sacrificed 96 h post inoculation and the cfu in the liver, spleen, uterus and colonic contents were enumerated as previously described [11].
T, NKT-like and NK cell percentages in blood from COPD patients and healthy controls were enumerated as previously reported [ 10].
Direct enzyme-linked immunospots (ELISPOTs) were enumerated as described previously [18] using a modification a protocol for indirect ELISPOTs [19].
The 95% confidence intervals are not drawn as previously, but are enumerated thereafter in square brackets.
Additionally, at each time point, several wells were stained using Trypan Blue (Sigma: St . Louis MO) and live cells were enumerated to determine viability, as previously described [ 30, 31].
Eosinophils were enumerated in formalin-fixed, H&E stained tissue sections as previously described (KC, SS, JM, Denver; HM, Chicago).
Circulating endothelial cells were enumerated by using four-colour flow cytometry as described previously (Beaudry et al, 2005; Norden-Zfoni et al, 2007; Mancuso et al, 2009).
23 The parameters measured in serum are enumerated in Table 1 and the procedures used have been previously described.
Tautomers, stereoisomers, and protonation states were enumerated and converted to a suitable database format as described previously.
